Past Issue

Volume: 11, Supplement: 1, Autumn 2009(Presented at 10th Royan Congress on Reproductive Biomedicine 5th Royan Congress on Stem Cell Biology & Technology) Pages: 0-0

premature LH Surge in Intrauterine Insemination (IUI) Cycles and GnRH Antagonist Rule

Objective: To assessment the usefulness of premature LH surge prevention in intrauterine insemination cycle by GnRH antagonist administration. Materials and Methods: 60 patients with unexplained or mild male infertility or minimal to mild endometriosis were enrolled to this prospective randomized controlled trial. 20 patients in group A (with GnRH antagonist) and 40 patients in group B (without GnRH antagonist) In all of participants CC+HMG were used for ovarian stimulation and when at least one follicle with ≥ 16 mm diameter was seen LH surge checked by urinary LH kit; in patients with negative results HMG was continued but in group A 0.25 mg Ganirelix SQ was administrated for two days then in both groups HCG was injected on the third day and IUI was done 36-40 hours later. Pregnancy was the primary outcome. Results: Base line characters and clinical parameters were similar in both groups except ≥18 mm follicles in group A (p value= 0.003). pregnancy rate in both group was not significantly different (10% in group A and 15% in group B). Conclusion: At least in CC+HMG stimulated cycles for IUI the occurrence of premature LH surge could be useful and GnRH antagonist administration could have an interventional mistiming rule. Keywords: IUI GnRH Antagonist Urinary LH kit P-46: The Effects of Electromagnetic Field on Mouse Blastocyst Apoptosis Rajaei F1* Borhani N2 Shams A.R2 1. Infertility Centre Qazvin University of Medical Sciences Qazvin Iran 2. Anatomy Qazvin University of Medical Sciences Qazvin Iran Email: Objective: The growing development of electronic industries and the increasing use of electrical appliances have led to higher rise in chronic exposure of people to extremely-low-frequency electromagnetic field (ELF-EMF). The aim of the present study was to investigate the possible effect of electromagnetic field exposure on DNA fragmentation in mouse blastocysts Materials and Methods: Eighty female NMRI mice were randomly divided into 2 groups of 40 animals: Control group was left unexposed. EMF group was exposed to 50 Hz & 0.5 mT EMF for 4 hours per day 6 days a week and a total duration of 2 weeks. On the 8th day of exposure the female mice in both groups were superovulated and mated overnight. In the next morning the female mice with a vaginal plug were identified as pregnant; at the time of implantation the pregnant mice were killed and blastocysts obtained by flushing the uterus horns. The mean number of pregnant mice blastocysts after flushing and the DNA fragmentation index following TUNEL staining in both groups were compared using statistical methods (SPSS t test p