Current Issue

Volume 19, Number 2, Summer 2017, Serial Number: 74 Pages: 269-277

Synthesis and Characterization of PMBN as A Biocompatible Nanopolymer for Bio-Applications


Puria Motamed Fath, M.Sc, 1, *, Fatemeh Yazdian, Ph.D, 1, Rogayyeh Jamjah, Ph.D, 2, Bahman Ebrahimi Hosseinzadeh, Ph.D, 1, Maede Rahimnezhad, M.Sc, 1, Razi Sahraeian, Ph.D, 2, Ashrafalsadat Hatamian, Ph.D, 1,
Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Iran Polymer and Petrochemical Institute, Tehran, Iran
*Corresponding Address: P.O.Box: 14399-55941 Faculty of New Sciences and Technologies University of Tehran Tehran Iran Email:bahman.ebrahimi@ut.ac.ir

Abstract

Objective

Poly [2-methacryloyloxyethyl phosphoryl choline (MPC)-co-n-buthyl methacrylate (BMA)-co-p-nitrophenyl-oxycrabonyl poly ethylene glycol-methacrylate (ME- ONP)] (PMBN), a biocompatible terpolymer, is a unique polymer with applications that range from drug delivery systems (DDS) to scaffolds and biomedical devices. In this research, we have prepared a monomer of p-nitrophenyl-oxycarbonyl poly (ethylene glycol) methacrylate (MEONP) to synthesize this polymer. Next, we designed and prepared a smart, water soluble, amphiphilic PMBN polymer composed of MPC, BMA, and MEONP.

Materials and Methods

In this experimental study, we dissolved MPC (4 mmol, 40% mole fraction), BMA (5 mmol, 50% mole fraction), and MEONP (1 mmol, 10% mole fraction) in 20 ml of dry ethanol in two necked flasks equipped with inlet-outlet gas. The structural characteristics of the synthesized monomer and polymer were determined by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (H-NMR), dynamic light scattering (DLS), gel permeation chromatography (GPC), scanning electron microscope (SEM), and transmission electron microscope (TEM) analyses for the first time. We treated the polymer with two different cell lines to determine its biocompatibility.

Results

FT-IR and H-NMR analyses confirmed the synthesis of the polymer. The size of polymer was approximately 40 nm with a molecular weight (MW) of 52 kDa, which would be excellent for a nano carrier. Microscopic analyses showed that the polymer was rodshaped. This polymer had no toxicity for individual cells.

Conclusion

We report here, for the first time, the full properties of the PMBN polymer. The approximately 40 nm size with an acceptable zeta potential range of -8.47, PDI of 0.1, and rod-shaped structure indicated adequate parameters of a nanopolymer for nano bioapplications. We used this polymer to design a new smart nano carrier to treat leukemia stem cells based on a target DDS as a type of bio-application.