Past Issue

Volume 19, Number 4, Jan-Mar(Winter) 2018, Serial Number: 76, Pages: 578-584

Mouse Bone Marrow-Derived Mast Cells Induce Angiogenesis by Tissue Engineering in Rats: Histological Evidence


Ali Karimi, M.Sc, 1, Rasoul Shahrooz, D.V.M., D.V.Sc., 1, *, Rahim Hobbenaghi, D.V.M., D.V.Sc., 2, Rahim Mohammadi, D.V.M., D.V.Sc., 3, Esmaeil Mortaz, Ph.D., 4, 5,
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Massih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, Netherlands
*Corresponding Address: P.O. BOX: 57153-1177 Department of Basic Sciences Faculty of Veterinary Medicine Urmia University Urmia Iran Email:r.shahrooze@urmia.ac.ir

Abstract

Objective

Therapeutic angiogenesis is employed to induce vascular network formation and improve functional recovery in ischemia. The aim of this study is to find an appropriate method to recover local ischemic conditions.

Materials and Methods

In this experimental survey, 20 male Wistar rats weighing approximately 200-250 g were randomly divided into four experimental groups respectively: ischemia group in which the femoral artery was transected; phosphate buffer solution group (PBS) in which the femoral artery transected location was immersed with PBS; chitosan (CHIT) group in which the transected location was immersed in a 50 µL CHIT solution; and mast cell transplanted group in which the transected location was immersed with a mixture of 50 µL CHIT and 50 µL PBS that contained 1×106 mast cells.

Results

On day 14 after surgery, mean numbers of blood vessels of different sizes in the CHIT/mast cell group significantly increased compared to the other experimental groups (P<0.05).

Conclusion

Our data suggest that mast cell reconstitution could offer a new approach for therapeutic angiogenesis in cases of peripheral arterial diseases.