The Expression of TLR2 and TLR3 in Sertoli Cells
of Azoospermic Patients
Toll-like receptors (
Materials and Methods
In this experimental study, testicular biopsies were taken
from ten azoospermic men. Following enzymatic dissociation, the samples were
moved to lectin coated petri dishes. After a few passages, all cells were cultivated
and Seroli cells were sorted by flow cytometry. To confirm Sertoli cell purification, alkaline phosphatase activity (ALP) and immunohistochemistry assays were employed.
The expression of
Isolation, purification and cultivation of human Sertoli cells were performed
successfully. Efficacy of purification of Sertoli cells by fluorescence-activated cell sorting (FACS) sorter was ~97%. The type of cultured cells was confirmed by vimentin and
follicle-stimulating hormone (FSH) receptor markers. Furthermore, the existence of anti-
Müllerian hormone in culture was confirmed. RT-PCR showed that both genes were expressed in Sertoli cells. Consistently, proteins of both were also expressed in Sertoli cells.
Moreover, QPCR showed that the relative expression of
This study confirmed expression of