Adipose Derived Stem Cells Affect miR-145 and p53 Expressions
of Co-Cultured Hematopoietic Stem Cells
Umbilical cord blood is used for transplantation purposes in regenerative medicine of hematological
disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological
processes such as cancer development and incidence. There is a new relation between
Materials and Methods
In this experimental study, we cultured passage-3 isolated human ADSCs as a feeder layer. Flow
cytometry analysis confirmed the presence of ADSC surface markers CD73, CD90, CD105.
ADSCs tested positive for mesenchymal stem cell (MSC) markers CD105, CD90, and CD73, and negative for HSC
markers CD34 and CD45. Our data demonstrated the differentiation potential of ASCs to osteoblasts by alizarin red and
alkaline phosphatase staining. MTT assay results showed a higher proliferation rate of CD34+cells directly cultured on the
ADSCs feeder layer group compared to the other groups. Direct contact between HSCs and the feeder layer was prevented
by a microporous membrane
ADSCs might increase HSCs proliferation and self-renewal through miR-145, p53, and their relationship.