Current Issue

Volume 19, Number 3, Oct-Dec (Autumn) 2017, Serial Number: 75 Pages: 512-519

Taraxerol Induces Cell Apoptosis through A Mitochondria-Mediated Pathway in HeLa Cells


Xiangyang Yao, Ph.D, 1, Binyu Lu, B.Sc, 2, Chaotian , Ph.D, 1, Qin Bai, B.Sc, 1, Dazhong Yan, Ph.D, 3, Hui Xu, M.Sc, 1, *,
Department of Biology and Food Engineering, Bengbu University, Bengbu, China
School of Pharmacy, Fudan University, Shanghai, China
School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
*Corresponding Address: Department of Biology and Food Engineering Bengbu University No. 1866 Caoshan Road Bengbu 233030 China Email:leeyxy@gmail.com

Abstract

Objective

Taraxerol acetate has potent anti-cancer effects via the induction of apoptosis, autophagy, cell cycle arrest, and inhibition of cell migration. However, whether taraxerol induced apoptosis and its underlying mechanisms of action is not clear. In the present study, we assess the effects of taraxerol on the mitochondrial apoptotic pathway and determine the release of cytochrome c to the cytosol and activation of caspases.

Materials and Methods

In this experimental study, we mainly investigated the effect of taraxerol on HeLa cells. We tested cell viability by the MTT assay and morphologic changes, analyzed apoptosis by DAPI staining and flow cytometry. We also determined reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) using a Microplate Reader. In addition, the apoptotic proteins were tested by Western blot.

Results

Taraxerol enhanced ROS levels and attenuated the MMP (Δψm) in HeLa cells. Taraxerol induced apoptosis mainly via the mitochondrial pathway including the release of cytochrome c to the cytosol and activation of caspases 9 and 3, and anti-poly (ADP- ribose) polymerase (PARP). Taraxerol could induce the down-regulation of the anti-apoptotic protein Bcl-2 and up-regulation of pro-apoptotic protein Bax. It suppressed the PI3K/ Akt signaling pathway.

Conclusion

These results demonstrated that taraxerol induced cell apoptosis through a mitochondria-mediated pathway in HeLa cells. Thus, taraxerol might be a potential anticervical cancer candidate.