Evidence of Oocyte Polarity in Bovine; Implications for
Intracytoplasmic Sperm Injection and Somatic
Cell Nuclear Transfer
We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine.
Materials and Methods
In this experimental study,
The proportional amount of maternal mRNA in S oocyte fragment was estimated
to be 6 to 11-fold higher than NS and FS counterparts. The relative abundances
The observation of spatial restriction of mRNAs and SEP within MII-oocyte may indicate that the principal forces of oocyte polarity are evolutionary conserved. This may in turn highlight the need for refinements in the methodology of intracytoplasmic sperm injection (where a sperm is injected far from the MII-spindle) and somatic cell nuclear transfer (where a major amount of regulative mRNAs that are associated with MIIspindle is removed during enucleation).