Current Issue

Volume 20, Number 1, Spring 2018, Serial Number: 77 Pages: 108-112

Evaluating The Effect of Melatonin on HAS2 , and PGR Expression, as Well as Cumulus Expansion, and Fertility Potential in Mice


Maryam Ezzati, M.Sc, 1, 2, Leila Roshangar, Ph.D, 1, 3, *, Jafar Soleimani Rad, Ph.D, 2, 3, Nahid Karimian, M.Sc, 4,
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Tissue Engineering, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Advanced Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Address: P.O. BOX: 51376563833 Stem Cell Research Center Tabriz University of Medical Sciences Tabriz Iran Email:lroshangar@yahoo.com

Abstract

Objective

Infertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is melatonin. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if melatonin could regulate hyaluronan synthase-2 (HAS2 ) and Progesterone receptor (PGR ) expressions in the cumulus cells of mice oocytes and provide an in vitro fertilization (IVF) approach.

Materials and Methods

In this experimental study, for this purpose, 30 adult female mice and 15 adult male mice were used. The female mice were superovulated using 10 U of pregnant mare serum gonadotropin (PMSG) and 24 hours later, 10 U of human chorionic gonadotropin (hCG) were injected. Next, cumulus oocyte complexes (COCs) were collected from the oviducts of the female mice by using a matrix-flushing method. The cumulus cells were cultured with melatonin 10 µM for 6 hours and for real-time reverse transcription-polymerase chain reaction (RT-PCR) was used for evaluation of HAS2 and PGR expression levels. The fertilization rate was evaluated through IVF. All the data were analyzed using a t test.

Results

The results of this study showed that HAS2 and PGR expressions in the cumulus cells of the mice receiving melatonin increased in comparison to the control groups. Also, IVF results revealed an enhancement in fertilization rate in the experimental groups compared to the control groups.

Conclusion

To improve the oocyte quality and provide new approaches for infertility treatment, administration of melatonin as an antioxidant, showed promising results. Thus, it is concluded that fertility outcomes can be improved by melatonin it enhances PGR .