Past Issue

Volume 20, Number 1, Spring 2018, Serial Number: 77 Pages: 84-89

miR-31 and miR-145 as Potential Non-Invasive Regulatory Biomarkers in Patients with Endometriosis


Oranous Bashti, Ph.D, 1, Mehrdad Noruzinia, M.D.,Ph.D, 1, *, Masoud Garshasbi, Ph.D, 1, Morteza Abtahi, M.D, 2,
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Dena Hospital, Shiraz, Iran
*Corresponding Address: P.O. BOX: 14115-335 Department of Medical Genetics Faculty of Medical Sciences Tarbiat Modares University Tehran Iran Email:noruzinia@modares.ac.ir

Abstract

Objective

Endometriosis is a prevalent gynecologic disease affecting 10% of women in reproductive age. Endometriosis is diagnosed by laparoscopy that was followed by histologic confirmation. Early diagnosis will lead to a more effective treatment with much less morbidity. As miR-31 and miR-145 are shown to be directly or indirectly correlated to biological processes involved in endometriosis, the aim of this study was to examine the association of miR-31 and miR-145 expression in plasma with the presence of endometriosis.

Materials and Methods

In this case control study, the plasma samples of 55 patients with endometriosis and 23 women without endometriosis were collected, extracted and analyzed by real time quantitative polymerase chain reaction (qPCR) for the expression of miR-145 and miR-31.

Results

Our findings showed that miR-31 expression levels in stage 3 or 4 and stage 1 or 2 were significantly down- regulated (less than 0.01-fold, P<0.05), while the expression level of miR-145 was significantly up-regulated in women with endometriosis in stage 1 or 2.

Conclusion

Different cellular biological processes, such as differentiation, proliferation, mitochondrial function, reactive oxygen species (ROS) production, invasion and decidualization, are deregulated in endometriosis. miR-31 and miR-145 are microRNAs (miRNAs) with potential roles, as shown in pathologies like cancers. We found that miR- 31 was under-expressed in patients with endometriosis, while miR-145 was over-expressed in stage 1 or 2, indicating that they were relatively down-regulated in the more severe forms. Our findings suggested that these two miRNAs may be considered as potential biomarkers with probable implications in early diagnosis and even follow-up of patients with endometriosis.