Past Issue

Volume 19, Number 2, Jul-Sep (Summer) 2017, Serial Number: 74 Pages: 292-305

Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats


Hamed Rezaie Agdam, D.V.M, 1, Mazdak Razi, Ph.D, 2, *, Amir Amniattalab, D.V.Sc, 3, Hassan Malekinejad, Ph.D, 4, Morteza Molavi, D.V.M, 1,
Private Veterinary Practitioner, Urmia, Iran
Department of Comparative Histology, Faculty of Veterinary Medicine, Urmia, Iran
Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia, Iran
*Corresponding Address: P.O. Box: 1177 Department of Comparative Histology Faculty of Veterinary Medicine Urmia Iran Email:mazdak.razi@gmail.com

Abstract

Objective

Atrazine (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and sperm parameters after exposure to ATZ in rats.

Materials and Methods

In this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 µg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01.

Results

Co-administration of VitE and T significantly (P<0.05) increased ATZ-decreased TAC and TTM levels and reduced ATZ-increased MDA content. T and VitE significantly (P<0.05) increased serum levels of ATZ-reduced T (1.94 ± 0.96), IN-B (122.10 ± 24.33) and LH (0.40 ± 0.10). The T+VitE animals showed a reduction in apoptotic cells and an increase in Leydig cells steroidogenesis. Co-administration of T and VitE significantly (P<0.05) reduced the ATZ-induced DNA disintegrity and chromatin de-condensation. VitE and T protected germinal cells RNA and protein contents against ATZ-induced damages.

Conclusion

T and VitE in simultaneous form of administration were able to normalize the ATZ-induced derangements through promoting antioxidant capacity and endocrine function.