Induction of Apoptosis in Cancer Cells of pre-B ALL Patients
after Exposure to Platelets, Platelet-Derived
Microparticles and Soluble CD40 Ligand
Materials and Methods
In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with Plts, Plt-derived microparticles (Plt-MPs) as well as purified soluble CD40L (sCD40L). After 48 hours of co-culture, the anti-cancer activity of the aforementioned factors was surveyed using examination of apoptosis markers of the cells including active caspase-3 and CD95 using ELISA and flow cytometer techniques, respectively. Additionally, staining of the cells with 7-Aminoactinomycin D (7-AAD) was evaluated by flow cytometer technique. Trypan blue exclusion test and WST-1 method were also used to compare the death/survival status of the cells.
Levels of CD95 and caspase-3 were significantly increased in the all treated groups (P<0.05). On the other hand, trypan blue, 7-AAD and WST-1 methods showed significantly lower number of the live cells in the treated groups (P<0.05).
This study can show the ability of Plts, Plt-MPs and sCD40L for the induction of apoptosis in PBMCs of
pre-B-ALL patients. Further studies are necessary to elucidate the different effects of platelets on cancer cells