Past Issue

Volume 20, Number 3, Autumn 2018, Serial Number: 79, Pages: 348-354

Conditioned Medium Protects Dopaminergic Neurons in Parkinsonian Rats


Malihe Nakhaeifard, M.Sc, 1, Maryam Haji Ghasem Kashani, Ph.D, 1, 2, *, Iran Goudarzi, Ph.D, 1, Arezou Rezaei, Ph.D, 2,
School of Biology, Damghan University, Damghan, Iran
Institute of Biological Sciences, Damghan University, Damghan, Iran
*Corresponding Address: P.O.Box: 3671641167 School of Biology Damghan University Damghan Iran Email:kashani@du.ac.ir

Abstract

Objective

Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASC- CM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH+) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA).

Materials and Methods

In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 µg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively. Another groups received intravenous injections of 3×106 cells (ASCs group), 500 µl of CM (ASC-CM group) or medium [α-minimal essential medium (α-MEM) group)]. All rats underwent evaluations with the rotarod and apomorphine-induced rotation tests at 2, 4, 6, and 8 weeks post-injection. At 8 weeks we sacrificed some of the animals for real-time polymerase chain reaction (PCR) analysis, and evaluation of TH+cell counts.

Results

We observed a significant decrease in contralateral turns to the lesions in the ASCs and ASC-CM groups compared to the neurotoxin lesioned or α-MEM groups at 8 weeks post transplantation. Cell and CM- injected rats showed a significant increase of staying on the rotarod compared to the lesion or α-MEM groups. Cell and CM-treated rats showed significant increases in the NGF and NT3 genes, respectively, compared with the lesion group. Both treated groups showed significant increases in BDNF gene expression and TH+ cell density.

Conclusion

The results suggested that ASCs and ASC-CM protected dopaminergic neurons through the expressions of neurotrophin genes.