The Effects of Sodium Selenite on Mitochondrial DNA Copy
Number and Reactive Oxygen Species Levels of In Vitro
Matured Mouse Oocytes
The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels.
Materials and Methods
In this experimental study germinal vesicle (GV), metaphase I (MI), and metaphase II (MII)
stage oocytes were recovered from 6-8 week old female mice after superovulation. Some of the GV oocytes were
cultured and matured in the presence and absence of SS. Then
The maturation rate of GV oocytes to the MII stage significantly increased in the SS supplemented group
(79.25%) compared to the control group (72.46%, P<0.05). The intensity of mitochondrial staining was not different
among the studied groups, whereas the mitochondria distribution in the cytoplasm of the IVM oocytes showed some
aggregation pattern. The
SS increased oocyte mtDNA copy number by decreasing oxidative stress. SS had an association with better oocyte developmental competence.