Past Issue

Volume 20, Number 3, Autumn 2018, Serial Number: 79, Pages: 388-395

Metabolic Signature of Pluripotent Stem Cells


Sara Taleahmad, Ph.D, 1, 2, Seyedeh Nafiseh Hassani, Ph.D, 2, Ghasem Hosseini Salekdeh, Ph.D, 1, 3, *, Hossein Baharvand, Ph.D., 2, 4, *,
Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
*Corresponding Addresses: P.O.Box: 16635-148 Department of Molecular Systems Biology Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran P.O.Box: 16635-148 Department of Stem Cells and Developmental Biology Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Emails:Salekdeh@RoyanInstitute.org,Baharvand@RoyanInstitute.org

Abstract

Objective

Pluripotent stem cells (PSCs), with the capacity to self-renew and differentiate into all other cell types, are of benefit in regenerative medicine applications. Tightly controlled gene expression networks and epigenetic factors regulate these properties. In this study, we aim to evaluate the metabolic signature of pluripotency under 2i and R2i culture conditions versus serum condition.

Materials and Methods

In this experimental study, we investigated bioinformatics analysis of the shotgun proteomics data for cells grown under 2i, R2i, and serum culture conditions. The findings were validated by cell cycle analysis and gene expressions of the cells with flow cytometry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively.

Results

Expressions of 163 proteins increased in 2i-grown cells and 181 proteins increased in R2i-grown cells versus serum, which were mostly involved in glycolysis signaling pathway, oxidation-reduction, metabolic processes, amino acid and lipid metabolism. Flow cytometry analysis showed significant accumulation of cells in S phase for 2i (70%) and R2i (61%) grown cells.

Conclusion

This study showed that under 2i and R2i conditions, glycolysis was highlighted for energy production and used to maintain high levels of glycolytic intermediates to support cell proliferation. Cells grown under 2i and R2i conditions showed rapid cell cycling in comparison with the cells grown under serum conditions.