Current Issue

Volume 20, Number 4, Jan-Mar(Winter) 2019, Serial Number: 80 Pages: 564-568

Association of Increased Levels of lncRNA H19 in PBMCs with Risk of Coronary Artery Disease


Sara Bitarafan, M.Sc, 1, Mohsen Yari, M.Sc, 1, Mohammad Ali Broumand, Ph.D, 2, Sayyed Mohammad Hossein Ghaderian, Ph.D, 1, Mahnoosh Rahimi, M.Sc., 1, 3, Reza Mirfakhraie, Ph.D., 1, Faezeh Azizi, Ph.D, 1, Mir Davood Omrani, Ph.D, 1, *,
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Molecular Pathology, Tehran Heart Center Tehran University of Medical Sciences, Tehran, Iran
Department of Bioinformatics and Genomics, Pharmacogenetic Research Center, Simple LIMS, San Diego, CA, USA
*Corresponding Address: P.O.Box: 19857-17443 Department of Medical Genetics Faculty of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran Email:davood_omrani@sbmu.ac.ir

Abstract

Objective

Considerable research shows that long non-coding RNAs, those longer than 200 nucleotides, are involved in several human diseases such as various cancers and cardiovascular diseases. Their significant role in regulating the function of endothelial cells, smooth muscle cells, macrophages, vascular inflammation, and metabolism indicates the possible effects of lncRNAs on the progression of atherosclerosis which is the most common underlying pathological process responsible for coronary artery disease (CAD). The aim of present study was to assess whether the expression of the lnc RNA H19 was associated with a susceptibility to CAD by evaluating the expression level of H19 in the peripheral blood.

Materials and Methods

A case-control study of 50 CAD patients and 50 age and sex-matched healthy controls was undertaken to investigate whether the H19 lncRNA expression level is associated with a CAD using Taqman Real-Time polymerase chain reaction (PCR).

Results

The subsequent result indicated that the H19 lncRNA was over-expressed in CAD patients in comparison with the controls. However, it was not statistically significant. This overexpression may be involved in coronary artery disease progression.

Conclusion

We report here, the up-regulation of H19 lncRNA in the whole blood of CAD patients and suggest a possible role for H19 in the atherosclerosis process and its consideration as novel biomarker for CAD.