Department of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR,
Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
Department of Neurology, School of Medicine, Mostafa Khomeini Hospital, Shahed University, Tehran, Iran
Iranian Biological Resource Center (IBRC)
Shahid Sabonchi St
Shahid beheshti Ave
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Chavoshi TarzjaniSeyedeh Parisa,
FazeliSeyed Abolhassan Shahzadeh,
Heat Shock Protein 70 and The Risk of Multiple Sclerosis
in The Iranian Population .
Multiple sclerosis (MS) is a chronic disease of the central nervous system and one of the most common causes of neurological
disability among those aged 20-40 years, particularly in women. Major histocompatibility complex (MHC) Class II genes
are known to be involved in the development of MS. One of the important groups of this complex is the HSP gene family,
especially HSP70, which is induced under stress conditions. The aim of the present case-control study was to determine
the association between the heat shock protein 70 (HSP70) and risk of MS in Iranian patients by genotyping the rs1061581
gene polymorphism. A total of 50 relapsing-remitting MS (RRMS) patients and 50 healthy control subjects were considered
for this study. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-
RFLP) method. PCR-RFLP results of twenty-five randomly selected samples were confirmed by DNA sequencing. Genotypic
and allelic distributions were compared between the case and control groups. We observed no significant difference in the
distribution of rs1061581 genotype and allele frequencies between RRMS patients and controls. In addition, there was no
association between the HSP70 gene polymorphism and the clinical variables in the case group. Our data indicate that
HSP70, in particular rs1061581, is unlikely to be involved in the susceptibility to or the severity of RRMS in Iranian patients.
Further large prospective studies are required to confirm these findings.