Differential Proliferation Effects after Short-Term Cultivation of
Mouse Spermatogonial Stem Cells on Different Feeder Layers
Spermatogonial stem cells (SSCs) provide the cellular basis for sperm production transforming the male’s genetic information to the next generation. We aimed to examine the effect of different feeder layer on proliferation of SSCs.
Materials and Methods
In this experimental study, we compared the
In our study, we observed a significant difference in the proliferation rates and colony size of SSCs among
the groups, especially for MEFs (P<0.05). SSCs can proliferate on MEFS, but not on STO, neonate or adult TSCs.
Using immunocytochemistry by KI67 the proliferative activities of SSC colonies on MEFs were confirmed. The results
of Fluidigm real-time polymerase chain reaction (RT-PCR) showed a high expression of the germ cell genes the
promyelocytic leukemia zinc finger protein (
Based on the optimal effect of MEFs for better colonization of SSCs, these feeder cells seem to be appropriate candidates for SSC cultures prior to transplantation. Therefore, it is suggested using these feeder cells for SSC cultivation.