Mitochondrial Polymorphisms, in The D-Loop Area, Are Associated with Brain Tumors


Donya Altafi, M.Sc, 1,*Soha Sadeghi, M.Sc, 1Hamed Hojatian, M.Sc, 1Maryam Torabi Afra, M.Sc, 1Safoura Pakizeh Kar, M.Sc, 2Mojtaba Gorji, Ph.D, 3Massoud Houshmand, Ph.D, 4,5,*
Molecular Biology Department, NourDanesh Institute of Higher Education, Esfahan, Iran
Department of Biology, International University of Guilan, Guilan, Iran
Department of Hematology and Oncology, Lorestan Medical University, Lorestan, Iran
Department of Medical Genetics, National Institutes for Genetic Engineering and Biotechnology, Tehran, Iran
Research Center, Knowledge University, Erbil, Kurdistan Region, Iraq
Molecular Biology Department, NourDanesh Institute of Higher Education, Esfahan, Iran
Department of Biology, International University of Guilan, Guilan, Iran
Department of Hematology and Oncology, Lorestan Medical University, Lorestan, Iran
Department of Medical Genetics, National Institutes for Genetic Engineering and Biotechnology, Tehran, Iran
Research Center, Knowledge University, Erbil, Kurdistan Region, Iraq
*Corresponding Addresses: P.O.Box: 1483614681 Molecular Biology Department NourDanesh Institute of Higher Education Esfahan Iran P.O.Box: 14965/161 Department of Medical Genetics National Institutes for Genetic Engineering and Biotechnology Tehran Iran Emails:donya.altafi@gmail.com,massoudh@nigeb.ac.ir
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Altafi Donya, Sadeghi Soha, Hojatian Hamed, Torabi Afra Maryam, Pakizeh Kar Safoura, Gorji Mojtaba, Houshmand Massoud. Mitochondrial Polymorphisms, in The D-Loop Area, Are Associated with Brain Tumors. Cell J. 2019; 21(3): 350-356.

Abstract

Objective

This study was carried out to evaluate the relationship between mtDNA D-loop variations and the pathogenesis of a brain tumor.

Materials and Methods

In this experimental study, 25 specimens of brain tumor tissue with their adjacent tissues from patients and 454 blood samples from different ethnic groups of the Iranian population, as the control group, were analysed by the polymerase chain reaction (PCR)-sequencing method.

Results

Thirty-six variations of the D-loop area were observed in brain tumor tissues as well as the adjacent normal tissues. A significant difference of A750G (P=0.046), T15936C (P=0.013), C15884G (P=0.013), C16069T (P=0.049), T16126C (P=0.006), C16186T (P=0.022), T16189C (P=0.041), C16193T (P=0.045), C16223T (P=0.001), T16224C (P=0.013), C16234T (P=0.013), G16274A (P=0.009), T16311C (P=0.038), C16327T (P=0.045), C16355T (P=0.003), T16362C (P=0.006), G16384A (P=0.042), G16392A (P=0.013), G16394A (P=0.013), and G16477A (P=0.013) variants was found between the patients and the controls.

Conclusion

The results indicated individuals with C16069T [odds ratio (OR): 2.048], T16126C (OR: 2.226), C16186T (OR: 3.586), G16274A (OR: 4.831), C16355T (OR: 7.322), and T16362C (OR: 6.682) variants with an OR more than one are probably associated with a brain tumor. However, given the multifactorial nature of cancer, more investigation needs to be done to confirm this association.