Transcription Levels of nicotinamide nucleotide transhydrogenase and Its Antisense in Breast Cancer Samples


Soraya Saleh Gargari, M.D, 1Mohammad Taheri, M.Sc, 2Vahid Kholghi Oskooei, Ph.D, 3Mir Davood Omrani, Ph.D, 2,3,*Soudeh Ghafouri-Fard, Ph.D, 3,*
Feto-Maternal Unit, Shohadaye Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Feto-Maternal Unit, Shohadaye Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
*Corresponding Address: P.O.Box: 19857-17443 Department of Medical Genetics Shahid Beheshti University of Medical Sciences Tehran Iran Emails:davood_omrani@yahoo.co.uk,s.ghafourifard@sbmu.ac.ir
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Saleh Gargari Soraya, Taheri Mohammad, Kholghi Oskooei Vahid, Omrani Mir Davood, Ghafouri-Fard Soudeh. Transcription Levels of nicotinamide nucleotide transhydrogenase and Its Antisense in Breast Cancer Samples . Cell J. 2019; 21(3): 331-336.

Abstract

Objective

To evaluate association of patients’ clinicopathological data with expression of nicotinamide nucleotide transhydrogenase (NNT) and naturally occurring antisense RNA of the same gene locus (NNT-AS1) in breast cancer samples.

Materials and Methods

In the current case-control study, mean expressions of NNT and NNT-AS1 were assessed in 108 breast tissue samples including 54 invasive ductal carcinoma samples and 54 adjacent non-cancerous tissues (ANCTs) by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).

Results

NNT expression was not significantly different between tumor tissues and ANCTs. However, NNT-AS1 expression was significantly down-regulated in tumor tissues compared to ANCTs (expression ratio=0.51, P=0.01). NNT-AS1 expression was significantly higher in estrogen receptor (ER) negative samples, in comparison with ER positives (P=0.01). No considerable difference was found in the gene expressions between other subcategories of patients. Considerable correlations were detected between expression levels of these two genetic loci in both tumor tissues and ANCTs.

Conclusion

In the current study, for the first time we simultaneously assessed expression of NNT and NNT-AS1 in breast cancer tissues. This study highlights association of ER status with dysregulation of NNT-AS1 in breast cancer tissues. Future researches are necessary to explore the function of this long non-coding RNA (lncRNA) in the pathogenesis of breast cancer.