Past Issue

Volume 15, Number 2, Summer 2013, Serial Number: 58, Pages: 142-151

Promotion of Remyelination by Adipose Mesenchymal Stem Cell Transplantation in A Cuprizone Model of Multiple Sclerosis


Azim Hedayatpour, Ph.D., 1, Iraj Ragerdi, Ph.D., 1, *, Parichehr Pasbakhsh, Ph.D., 1, Laya Kafami, Ph.D., 2, Nader Atlasi, M.Sc., 1, Vahid Pirhajati Mahabadi, Ph.D., 1, Soudabeh Ghasemi, B.Sc., 1, Mahmoudi Reza, Ph.D., 3,
Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Pathobiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
Department of Cellular and Molecular Research Center,Yasuj University of Medical Sciences,Yasuj, Iran
* Corresponding Address: P.O.Box: 1417613151 Department of Anatomical Sciences School of Medicine Tehran University of Medical Sciences TehranIran Email: ragerdi@sina.tums.ac.ir

Abstract

Objective:

Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system (CNS). Stem cell transplantation is a new therapeutic approach for demyelinating diseases such as MS which may promote remyelination. In this study, we evaluate the remyelinating potential of adipose mesenchymal stem cells (ADSCs) and their effect on neural cell composition in the corpus callosum in an experimental model of MS.

Materials and Methods:

This experimental study used adult male C57BL/6 mice. Cultured ADSCs were confirmed to be CD73+,CD90+, CD31-,CD45-, and labeled by PKH26. Animals were fed with 0.2% w/w cuprizone added to ground breeder chow ad libitum for six weeks. At day 0 after cuprizone removal, mice were randomly divided into two groups: the ADSCs-transplanted group and the control vehicle group (received medium alone). Some mice of the same age were fed with their normal diet to serve as healthy control group. Homing of ADSCs in demyelinated lesions was examined by fluorescent microscope. At ten days after transplantation, the mice were euthanized and their cells analyzed by luxol fast blue staining (LFB), transmission electron microscopy and flow cytometry. Results were analyzed by one-way analysis of variance (ANOVA).

Results:

According to fluorescent cell labeling, transplanted ADSCs appeared to survive and exhibited homing specificity. LFB staining and transmission electron microscope evaluation revealed enhanced remyelination in the transplanted group compared to the control vehicle group. Flow cytometry analysis showedan increase in Olig2 and O4 cells and a decrease in GFAP and Iba-1 cells in the transplanted group.

Conclusion:

Our results indicate that ADSCs may provide a feasible, practical way for remyelination in diseases such as MS.