Objective: Bone marrow stromal cells (BMSCs or MSCs) are adult stem cells that have the potential for transdifferentiation into different cell types and carry the potential for repairing degenerative injuries and diseases. For functional recovery by these cells, the grafted cells must secrete the required complement of factors for repair of the damaged region. BMSCs normally give rise to bone, cartilage, and mesenchymal cells. In recent years, bone marrow cells have been shown to have the capacity to differentiate into myocytes, hepatocytes, adipocytes, beta-pancreatic islets cells, glial cells and neural cells under specific experimental cell culture conditions by using appropriate growth factors. Recently it has shown that different condition of mechanical force and gravity can regulate differentiation of BMSCs through various signaling pathways, too. Materials and Methods: For hypergravity experiment, BMSCs were cultured on a cell centrifuger to obtain hypergravity and for simulating microgravity; cells were cultured on a clinostat. Then both samples used to differentiate into cardiomyocytes, osteoblasts and adipocytes. Results: Studies have shown that hypergravity promotes BMSC differentiate into force-sensitive cells, cardiomyocytes and osteoblasts, whereas microgravity promotes differentiation into force-insensitive cells, namely adipocytes. Also hypergravity promoted BMSCs proliferation and microgravity inhibited proliferation of them. Studies have shown that gravity condition affects mRNA expression, too. In BMSCs, hypergravity increase the expression of cardiomyocytes and osteoblasts markers but decrease the expression of the adipocytes markers. Conclusion: All together gravity is an important factor affecting the differentiation of BMSCs. This provides a new window for studies of stem cell differentiation and new approach in cell therapy and regenerative medicine.