Ps-49: Comparative Analysis of Growth Characterization of Stem Cells Derived from Human Dental Pulp Polyp (PPSCs) versus Normal Dental Pulp Stem Cells (DPSCs)


Hosseini SM *, Attar A , Tavangar MS , Baghaban Eslaminejad MR , Karamzadeh R ,

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Objective: Dental pulp stem cells (DPSCs) have been shown to have more replicative potentials in comparison to bone marrow mesenchymal stem cells (MSCs) while having the same differentiation capacity. We have previously investigated the isolation of stem cells from pulp polyps (chronic hyperplastic pulpitis), a pathological tissue produced in an inflammatory proliferative response within a tooth supplied by a very good blood flow. The Goal of this study was to assess growth characterization analysis of these cells and compare them to DPSCs. Materials and Methods: Pulp polyps of eight permanent molar teeth achieved through simple curettage of pulp chamber. Four samples of healthy wisdom dental pulps were taken as the control. The tissues were digested by collagenase and dispase. Single cells were harvested and cultured. To compare the growth characteristics of polyp derived stem cells and DPSCs, 3rd passage cells from healthy pulps and polyps were seeded with the concentration of 2×104 cells/well in all the wells of 12-well culture plates. Every day cells from one of the wells were detached by Trypsin-EDTA and counted with hemocytometer. Based on the results we draw growth curve and population doubling time was calculated by the formula “logN2-logN1/ log2” in which the N1 shows the number of cells in the beginning of the log phase of growth and N2 shows the number of cells at the end of the log phase. Results: Cells isolated from pulp polyps displayed similar features regarding morphological characteristics DPSCs do. The pulp polyp derived cells grew faster than DPSCs. The mean for population doubling time for pulp polyp derived cells is 1.13 days and for DPSCs is 1.57 days (p = 0.086). Conclusion: Pulp polyps are easily accessible noninvasively acquired tissue resources which contain adequate number of cells with a faster growth pattern in comparison to DPSCs.