Ps-56: Evaluation of Effect of Tail and Lung Tissue Extract on Differentiation of MSCs to Nerve Cells


Khanbabaee R *, Kasaian M , Shiravi AH ,

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Objective: Mesenchymal stem cells (MSCS) are important adult stem cells that attracted many researchers. Bone marrow derived MSCS have capable of differentiation to much kind of cells including fat cells, Osteocyte, Chondrocyte and Neuron. Most of substances that up to now used to induction of differentiation of stem cells to neural cells is: Retinoic acid, Dimethyl Solfuxid, Deprenil and ... that are toxic compounds or expensive. In this study, lung and tail tissue extracts of embryo is used as non-toxic inducing molecule. Materials and Methods: MSCS prepared and cultured from adult mouse femur (BALB / C). After four subcultures, lung and tail tissue extracts with density of 50%, 70% and 80% in group І and ІІ treated for 3 days and 7 days respectively. In morphological study, Krezil violet staining was carried out and in flowcytometry study, Nestin and Tubulin antibodies used for assess of molecular character of neurons. Results: The flowcytometric results showed that in group І, the Nestin marker expression level in contrast to control group decreased significantly (p < 0.05), but Tubulin marker expression increased significantly. In group ІІ, the expression levels of Nestin and Tubulin markers increased significantly in contrast to control group (p < 0.05). Results of Morphological and flowcytometric study were in line. Conclusion: Induction of MSCS with extracts of lung and tail tissue increased the differentiation of MSCS to neuron. This study showed that MSCs have the ability to differentiate to neurons in vitro and this induction depends on the type of inducer.