Ps-73: Long Term Storage and Banking of Stem cells Exist in Peripheral Blood


Mohammad M *, Mehdizadeh M , Mirmorsali L , Nouri M , Janzamin E , Sahraneshin Samani F , Ebrahimi M ,

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Objective: Freezing the peripheral cells is suitable therapeutic method and approach for long term storing to the patients suffering from cancer such as Multiple myeloma, Hodgkin's lymphoma, Non-Hodgkin's lymphoma and after the chemical therapy. This study presents the result of storing and transplantation of peripheral blood cells after four years. Materials and Methods: Donors were patients or their close relatives. Intravenous GCST 300 Mg/Kg (Neupogen) were injected for five days. Increasing of hematopoitic stem cells were followed by CBC count, then samples sent to Royan public cord blood bank. The number of cells stored in the bag was 6×109 ± 1. DMSO and DEXTRAN were used for freezing the peripheral samples. The number of cells, percentage of viability, percentage of CD34+, number of colonies and their types were evaluated before and after freezing. Results: 40 blood units were stored from 2008 to 2011. The average number of nucleolus cells (NC) was 66.1×109 ± 29.95. The number of CD34 cells was 20.24 × 109 ± 11.69 and CFC was 57.38 × 104. The result of melting three samples after one year being in frozen state showed that the number of living cells had decreased by living cells. In the meanwhile, the function of CD34+ cells does not change significantly. Conclusion: Transplantation on three patient suffering from uterine cancer, Hodgkin,s lymphoma, Non-Hodgkin's lymphoma, were successful and present while more than two years have passed, no disease return have occurred.