Objective: Simvastatin is known as a treatment for hypercholesterolemia, although recently it is shown that this drug is able to stimulate bone formation. The goal of this study was to show whether similar phenomenon can occur in dental tissues and simvastatin can stimulate the differentiation of cementoblasts from Periodontal Ligament (PDL) cells. Materials and Methods: Human PDL cells were cultured and treated with different concentrations of simvastatin (10-6, 10-7, 10-8 and 10-9 μg /μl). Then for cementobastic differentiation, expression of Bone Morphogenetic Protein-2 (BMP-2) and GLUT-1 was assessed using quantitative real time polymerase chain reaction (qRT-PCR) after 24, 48 and 72 post culture. Results: GLUT-1 and BMP-2 expression were significantly increased in cultures containing 10-6, 10-7, 10-8 and 10-9 μg /μl of simvastatin with its major effect in the concentration of 10-7 μg /μl and after 72 hours of culture. Conclusion: Optimum concentrations of simvastatin can accelerate the differentiation of PDL cells to cementoblasts and has the potential to be used in periodontal regeneration.