Ps-126: Efficiency of Dendrimer G6 in FITC Conjugated Micro RNA Transfection to Embryonic Stem Cells, Unrestricted Somatic Stem Cells and Mesenchymal Stem Cells


Pourasgari F *, Nomani A , Hafizi M , Soleimani M , Massumi M ,

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Objective: Gene delivery vectors are divided into two groups: viral and non-viral vectors. Viral vectors are efficient in delivering of a gene to the target cells although, there are problems related to the safety and large scale production. So, non-viral vectors including of chemical vectors are still attractive because they are not immunogene, so they are more safe and easy for preparation. Among chemical vectors, dendrimers are a system used for gene delivering into cells. In this study, efficiency of dendrimer G6 (as a nucleic acid porter) was assessed in transferring of scramble microRNA-FITC conjugated into human mesenchymal stem cells; cord blood derived unrestricted somatic stem cells (USSC) and mouse embryonic stem cells, in comparison to lipofectamine. Materials and Methods: To asset to a real statitistic range for micro RNA (miR) transfection, the cell transfected by FITC conjugated micro RNA. After 24, 48 and 72 hours the transfection efficiency was investigated by fluorescent microscopy, flow cytometry and MTT assay. Results: Flourescent microscopy showed green color inside the cells. Flow cytometric studies showed G6 has higher transfection efficiency in hMSCs, USSCs and the embryonic stem cells than lipofectamine. Although, cytotoxicity studies by MTT revealed that dendrimer G6 had a higher cytotoxicity effect than lipofectamine in miR transferring to all of the given cells. Conclusion: Therefore, in respect to efficiency of gene transferring and cost, our study introduces dendrimer G6 as an efficient carrier for miR transferring to different types of stem cells.