Objective: Acute hepatic failure (AHF) is a severe liver injury accompanied by sustained liver damage. Management of severe AHF is one of the most challenging problems in clinical medicine . It is imperative that new approaches for repairing the liver are developed. Cellbased therapy has been implicated in the treatment of liver diseases. Mesenchymal stem cells (MSCs) from various sources such as bone marrow is available. These cells is one of the major candidates in cell therapy. The production of IGF-I increases in the regenerating liver. Insulin-like growth factor – I (IGF-I) in liver regeneration is effective after binds to IGF-I receptor. Increasing expression of IGF-IR in tumor necrosis factor-α (TNF-α) - treated MSCs may be cause to improve liver regeneration. Materials and Methods: Bone marrow was aspirated from human normal donor after inform consent. Cells were isolated and cultured. Identification of cells with Flow cytometric analysis and functional testes were performed. Fourth passage cells were treated with TNF-α at different doses (1ng/ml and 10ng/ml) and incubated at different times (2,10,24 and 48 hours). IGF-IR gene expression was investigated Using Real time - polymerase chain reaction technique. Results: Flowcytometric analysis showed that MSCs towards CD90 marker positive while is negative with respect to CD45,CD80, CD40 markers. Functional test for MSC was demonstrated by adipocyte and osteocytestaining with Oil- Red and Alizarin Red respectively. Increased gene expression was demonstrated in TNF-α treated in comparison with untreated cells. Conclusion: Increase gene expression pattern of IGFIR in human MSCs may be used for clinical stem cell therapy in AHF.