Objective: Recently cell-based therapy approach seems to be a promising procedure for regeneration of bone defects. However finding the best cell source is challenging and every cell types have their pros and cons. Materials and Methods: In this study, the biomedical characteristic and osteogenic capability of the three stem cell groups containing mesenchymal stem cells isolated from bone marrow (BM-MSC), cord-blood-derived, unrestricted somatic stem cells (USSC) and adipose tissue (AT-MSC) are proposed as potential sources for cell therapy and functional tissue engineering. Results: The proliferation rate of the three named stem cells proliferation is the same to a great extent (p≤0.05). In osteogenic differentiation, alkaline phosphatase (ALP) activity reached the highest point in day 7 in unrestricted somatic stem cells, in comparison to mesenchymal stem cells isolated from bone marrow (BM-MSC) which displayed the most significant value of ALP activity in day 14. In whatever manner, during osteogenic induction, BM-MSC revealed the maximum ALP activity and mineralization .Moreover, adipose tissue stem cells presented the least capability for mineralization within differentiation and had the lowest ALP activity on days 7 and 14. Even supposing, the rate of expression for levels of osteonectin , collagen type I, and BMP-2 in undifferentiated state AT-MSC was significantly higher, but these genes were expressed sharply in BM-MSC during differentiation. In the incubation process, BM-MSC expressed the greatest amount of ALP, Runx2, and osteocalcin. Conclusion: All things considered, BM-MSC included the greatest potential for osteogenic differentiation and keep a valuable treasure in cell based therapies and tissue engineering applications.