Molecular Screening of Staphylococcal Enterotoxin B Gene in Clinical Isolates

(Pages: 187-193)
Ramezan Ali Ataee, Ph.D., 1,*Ali Karami, Ph.D., 2Morteza Izadi, M.D., 3Aref Aghania, M.Sc., 1Mohammad Hossein Ataee, M.Sc., 1
* Corresponding Address: P.O. Box: 19395-5487 Therapeutic Microbial Toxin Research Center Baqiyatallah University of Medical Sciences Mollasadra Ave., Tehran Iran Email:ataee@bmsu.ac.ir
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Ataee Ramezan Ali, Karami Ali, Izadi Morteza, Aghania Aref, Ataee Mohammad Hossein. Molecular Screening of Staphylococcal Enterotoxin B Gene in Clinical Isolates. Cell J. 2011; 13(3): 187-192.

Abstract

Objective: The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. Materials and Methods: In this experimentally study, 300 Staphylococcus aureus (S. aureus) strains isolated from clinical samples were assayed. The isolated strains were confirmed by conventional bacteriological methods. Polymerase chain reaction (PCR) was used to determine the enterotoxin B (ent B) gene. Assessment of toxin production in all strains that contained the ent B gene was then performed. Finally, using specific antibody against SEB, a Western-blot was applied to confirm detection of enterotoxin B production. Results: Results indicated that only 5% of the 300 clinically isolated S. aureus contained the ent B gene. All strains which contained the ent B gene produced a proteinous enterotoxin B. The results of sequence determination of the PCR product were compared with the gene bank database and 98% similarity was achieved. The results of the Western-blot confirmed that enterotoxin B was produced in strains that contained the ent B gene. Conclusion: The results of this study indicate that 5% of clinically isolated S. aureus strains produce enterotoxin B. Considering that the enterotoxin B is an important superantigen, it is possible that a delay in diagnosis and lack of early proper treatment can cause an incidence of late complications, particularly in staphylococcal chronic infections. For this reason, it is suggested that in addition to detecting bacteria, an enterotoxin B detection test should be performed to control its toxigenicity.