Prevention and Detection of Mycoplasma Contamination in Cell Culture


Laleh Nikfarjam, Ph.D., *Parvaneh Farzaneh, Ph.D.,
Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
* Corresponding Address: P.O.Box: 15855-161Human and Animal Cell Bank Iranian Biological Resource CenterACECR Tehran Iran Email: nikfarjam@ibrc.ir
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Abstract

One of the main problems in cell culture is mycoplasma infection. It can extensively affect cell physiology and metabolism. As the applications of cell culture increase in research, industrial production and cell therapy, more concerns about mycoplasma contamination and detection will arise. This review will provide valuable information about: 1. the ways in which cells are contaminated and the frequency and source of mycoplasma species in cell culture; 2. the ways to prevent mycoplasma contamination in cell culture; 3. the importance of mycoplasma tests in cell culture; 4. different methods to identify mycoplasma contamination; 5. the consequences of mycoplasma contamination in cell culture and 6. available methods to eliminate mycoplasma contamination. Awareness about the sources of mycoplasma and pursuing aseptic techniques in cell culture along with reliable detection methods of mycoplasma contamination can provide an appropriate situation to prevent mycoplasma contamination in cell culture.