Effects of Saffron (Crocus sativus L.) Aqueous Extract
on In vitro Maturation, Fertilization and Embryo
Development of Mouse Oocytes
Lower pregnancy rates of
Materials and Methods:
In this experimental study ,cumulus-oocyte complexes (COCs) were collected from 6-8 weeks old novel medical research institute (NMRI) female mice ovaries. COCs were cultured in IVM medium supplemented with 0 (control), 5, 10, 20 and 40 µg/ml of SAE in 5% CO2 at 37℃. The rates of maturation, fertilization and development were recorded. ANOVA and Duncan's protected least significant test, using the SAS program was applied for all statistical analysis.
The maturation rate was significantly higher in all groups treated with different
concentrations of SAE compared with the control group (p<0.05). However, the lower
concentrations of SAE (10 and 5 µg/ml) in maturation medium respectively increased the
fertilization rate of oocytes and
The results of this study indicate that lower concentrations of SAE are more appropriate to be added to maturation medium when compared with other experimental and control groups. Generally, we conclude that addition of appropriate amounts of natural extracts such as SAE to maturation medium improves oocyte maturation and embryo development.