Evaluation of Bcl-2 Family Gene Expression in Hippocampus of 3, 4-methylenedioxymethamphetamine Treated Rats

(Pages: 275-280)
Sara Soleimani Asl, Ph.D., 1Mohammad Hassan Farhadi, M.Sc., 2Kazem Moosavizadeh, Ph.D., 3Ali Samadi Kuchak Saraei, Ph.D., 3Mansoure Soleimani, Ph.D., 3,4Seid Behnameldin Jamei, Ph.D., 4Mohammad Taghi Joghataei, Ph.D., 3Alireza Samzadeh-Kermani, Ph.D., 5Hamed Hashemi-Nasl, M.Sc., 4Mehdi Mehdizadeh, Ph.D., 3,4,*
* Corresponding Address: P.O.Box 15875-1454 Cellular and Molecular Research Center Tehran University of Medical Sciences Tehran Iran Email: mehdizadehm@tums.ac.ir
Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Soleimani Asl Sara, Farhadi Mohammad Hassan, Moosavizadeh Kazem, Samadi Kuchak Saraei Ali, Soleimani Mansoure, Jamei Seid Behnameldin, Joghataei Mohammad Taghi, Samzadeh-Kermani Alireza, Hashemi-Nasl Hamed, Mehdizadeh Mehdi. Evaluation of Bcl-2 Family Gene Expression in Hippocampus of 3, 4-methylenedioxymethamphetamine Treated Rats. Cell J. 2012; 13(4): 275-280.

Abstract

Objective:

3,4-methylenedioxymethamphetamine (MDMA) is an illicit, recreational drug that causes cellular death and neurotoxicity. This study evaluates the effects of different doses of MDMA on the expression of apoptosis–related proteins and genes in the hippocampus of adult rats.

Materials and Methods:

In this expremental study,a total of 20 male Sprague Dawley rats (200-250 g ) were treated with MDMA (0, 5, 10, 20 mg/kg i.p. twice daily) for 7 days. Seven days after the last administration of MDMA, the rats were killed. Bax and Bcl-2 genes in addition to protein expressions were detected by western blot and reverse transcriptionpolymerase chain reaction (RT-PCR).Results were analyzed using one-way ANOVA and p≤0.05 was considered statistically significant.

Results:

Our results showed that MDMA caused dose dependent up-regulation of Bax and down-regulation of Bcl-2 in the hippocampus. There was a significant alteration in bcl-2 and bax genes density.

Conclusion:

Changes in apoptosis-related proteins and respective genes relating to Bax and Bcl-2 might be involved in the molecular mechanism of MDMA-induced apoptosis.