In the past decades, numerous progresses have been achieved in fabrication of tissues or organs using tissue Abstract of the 1st Int. Student Congress on Cell & Molecular Medicine Cell Journal(Yakhteh), Vol 12, Suppl 1, Winter 2011 17 engineering approach. It still remains challenging to create a tissue with identical composition and functional properties, especially the hierarchical structure as its native counterpart. The development of nanotechnology has opened a new avenue to tissue engineering regime, which allows the creation of cell-friendly microenvironment and therefore results in tissue formation with a more elaborate structure. Electrospun nanofibers are considered advantageous in regulating the cellular behavior due to their morphological similarity as native extracellular matrix. Its potential application for functional tissue formation has been proposed. We have explored the use of nanofibers for biomimetic creation of three-dimensional cell growing environment and generation of tissues with heterogeneous cells and composition. This biomimetic approach is subsequently evaluated for the creation of bone-like tissue and skin substitutes.