O-14: Evaluating the Regulatory Role of Mouse Oct-4 3’UTR in Bone Marrow Stem Cell (BMSC


Ghiasvand S *, Najafi R , Sadeghizadeh M ,

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Oct-4 also known as POU5F1 (POU domain, class 5, transcription factor 1) is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells such as tumor cells. Oct-4 expression must be closely regulated; too much or too little will actually cause differentiation of the cells. the level of Oct-4 expression in mice is vital for regulating pluripotency and early cell differentiation since one of its main functions is to keep the embryo from differentiating. there are two type of stem cell ;embryonic stem cells that are isolated from the inner cell mass of blastocysts, and adult stem cells that are found in adult tissues such as BMSC that are isolated from the bone marrow. translational control mechanisms result from the interaction of RNA-binding proteins and microRNA with 5' or 3'untranslated regions (UTRs) of mRNA. Two general mechanisms of translational inhibition by 3'UTR-binding proteins have been proposed, one in which mRNA is disrupted and another in which mRNA is required