O-19: Inhibitory Effect of RNA Interference on Tyrosinase Eexpression In vivo and In vitro (Pages: 26-27)

Ebrahimi A *, Mansouri N , Mehrabi A , Hedayati M ,


Tyrosinase mutation can induce human diseases, such as oculocutaneous albinism. Melanin is a heterogeneous polymer produced and concentrated Abstract of the 1st Int. Student Congress on Cell & Molecular Medicine Cell Journal(Yakhteh), Vol 12, Suppl 1, Winter 2011 27 within the melanosomes of the melanocytes, and tyrosinase is a key rate-limiting enzyme for melanin biosynthesis. It is a glycosylated protein and a major regulator of melanogenesis. To date, many approaches have been tried to regulate tyrosinase activity and melanin content. In the present study, we aimed to directly regulate tyrosinase expression using RNA interference (RNAi). We screened small interfering RNAs (siRNAs) corresponding to the tyrosinase sequence using the mouse melanoma cell line B16 and then delivered the most effective sequence into C57BL/6 mice to study its effect in vivo