O-29: Protective and Therapeutic Effects of Hexamethylenettramyn (HMT) on Tracheal Epithelial Cells in Rat Exposed to Sulfur Mustard


Ajudani R *, Kaka GhR , Sadraei SH , Salmasi S , Safarpour H ,

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Objective: Sulfur mustard (HD) is a vesicant chemical agent. Inhalation of sulfur mustard, a bifunctional alkylating agent that causes severe tracheal epithelium damage, is a significant threat to both military and civilian populations. In medicine, hexamethylenttramyn (HMT) is used as anti bacterial in urinary tract infection. HMT has been shown to protect human lung cells against sulfur mustard toxicity. The aim of this study was to evaluate the protective and therapeutic effects of HMT against the mustard that affects on tracheal epithelium in rat. Materials and Methods: Twenty Albino wistar adult male rats were used in this study. Animals were randomly divided into one control and three experimental groups including: the normal saline (NS) as control and HD, the group protected with HMT prior to exposure to HD and HMT Therapeutic groups after HD exposure. HD and NS was injected intra tracheal. Therapeutic groups received HMT for 14 days intra peritoneally. Animals were sacrificed in the end of 14th day of experiments with ether and the middle one-third of trachea removed, fixed and processed for histological study. Histological sectioned at 5 microns were then stained with hematoxylin - eosin (H&E). The number of tracheal epithelial cell nuclei and the epithelial tracheal thickness were measured by Motic software in all groups. Statistical analysis was performed by T-test and ANOVA using spss software. Results: Our results showed that mean number of tracheal epithelial cell nuclei in the HD group has significantly decreased when compared to NS group, In addition, the mean number of tracheal epithelial cell nuclei in both therapeutic and protective group has no significant differences with HD group. No significant decrease of thickness of tracheal epithelium was seen in HD group when compared to NS group. Protective group could partly prevent of reduction of tracheal epithelium thickness against the HD but this difference was not significant. Conclusion: The use of Hexamethylenettramyn before and a little after exposure to sulfur mustard could have protective role against the tracheal epithelium histological damage