O-41: The Effect of Adipose Tissue Derived Mesenchymal Stem Cells (ADMSCs) on Wound Healing of Diabetic Rat Model


Khosravi Maharlooei M *, Bagheri M , Solhjou Z , Moein Jahromi B , Akrami M , Rohani L , Moini Y ,

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Objective: Stem cells are a new hope to ameliorate delayed diabetic wound healing. The aim of this study was to evaluate adipose tissue derived mesenchymal stem cells (AD-MSCs) effect on wound healing in a diabetic rat model. Materials and Methods: Twenty six male Sprague-Dawley rats were caused to become diabetic by a single intraperitoneal injection of streptozotocin. Diabetic rats were divided into two equal groups randomly; control and treatment. Six rats served as non-diabetic (non-DM). Six weeks later, a full-thickness circular excisional wound was created on the dorsum of each rat. AD-MSCs, derived from abdominal adipose tissue of a male rat, were dissolved in 1cc phosphate buffer saline (PBS), and were injected intra-dermally around the wounds of treatment group. PBS was applied to control and non-DM groups. The wound area was measured every other day. After wound healing completion, full thickness skin samples (1×1 cm) were taken from the wound sites for evaluation of volume density of collagen fibers, length and volume density of vessels, and numerical density of fibroblasts by stereological methods. Results: AD-MSCs injection accelerated wound healing rate in diabetic rats, but did not increase length and volume density of the vessels and volume density of the collagen fibers. AD-MSCs decreased the numerical density of the fibroblasts. Conclusion: Local intra-dermal injection of AD-MSCs enhances diabetic wound healing in diabetic rats probably by other mechanisms rather than enhancing angiogenesis or accumulating collagen fibers