P-1: Smads Gene Expression in Chemical Injured People Airway Epithelium by Sulfur Mustard


Adelipour M *, Rezaei J , Imani Fooladi A , Nourani M ,

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Objective: Sulfur mustard is a chemical warfare agent developed during Wars in world. Its production is easy and its vesicant property has the highest military significance. Iraqi forces frequently used it against Iranian during Iran –Iraq war in the 1980–1988 years. Pulmonary disease was diagnosed as the most important disorder in individuals that exposed to sulfur mustard. Inflammation and repair in the airway result in airway remodeling that include excess of extracellular matrix deposition in the airway wall. Both of inflammatory cells and epithelial cells were hypothesized to contribute to airway remodeling choronic obstructive pulmonary disease (COPD). Some of Smad molecules family as downstream gene of TGF-b involved in airway remodeling due to perebronchial fibrosis. In the present study we compared the mRNA expression for Smad2 and Smad3 genes in lung biopsies of chemical-injured patients with non-injured patients. Materials and Methods: Expression of Smad2 and Smad3 in healthy and SM induced airway wall. In ten normal individuals and fifteen SM induced patients were comprised. Smads expression were evaluated quantitatively by RT-PCR followed bands densitometry. Results: Expression levels of Smad2 and Smad3 in SM exposed patients were upregulated but Smad2 was not significant (p=0.27) and Smad3 significantly upregulated (p=0.035). Conclusion: As we are aware, this is the first study about the induction of Smad molecules pathway in patients exposed to SM. We suggested that over expression of Smad molecules in COPD induced by SM may follow an inflammatory procedure in bronchial wall of these patients at mRNA levels