P-2: Proteomic Analysis of Atherosclerosis (Pages: 40-41)

Ahmadi M *, Ahmadi M , Haghjooyjavanmard Sh , Nili N ,


Objective: Atherosclerosis (AS) is the leading cause of death in developed countries. Different approaches have been applied for prediction and more efficient therapeutic strategies. Recent rapid advances in genomic and proteomic tools and techniques hold great promise for transforming the practice of cardiovascular medicine. Proteomic approaches recently have been applied to atherosclerosis identify proteins and pathways involved in the predisposition to and pathophysiology of atherosclerosis. The goal of our study was to set up the conditions for comparing differentially secreted proteins from carotid atherosclerotic plaques and control arteries as well as to identify different secreted proteins after treatment with pomegranate peel anthocyanins as a potent antioxidant source. Materials and Methods: Differentially secreted proteins were identified by 2-D electrophoresis in rabbits feeding normal diet, high-cholesterol diet and high-cholesterol diet with pomegranate peel anthocyanins(0.1% in drinking water). Results: Dramatic alterations in the protein expression profile of aorta tissue was detected after the development of AS disease in rabbits and important difference between negative group and two other groups was observed. POM extract had no effect in aortic protein secretion. Conclusion: Investigation of the relationship between the differentially expressed proteins and the initiation and development of the AS would be crucial for understanding of the mechanism(s) of the disease and possible discovery of novel diagnosis markers and therapeutic approaches. In this study we observed important differences in aortic proteins secretion during atherosclerosis in rabbits. This finding may have important implications for the prevention of atherosclerosis and therapeutic interventions