Suppressive Effects of Resveratrol Treatment on The Intrinsic Evoked Excitability of CA1 Pyramidal Neurons


Gholamhossein Meftahi, Ph.D, 1,2Zohreh Ghotbedin, Ph.D, 3Mohammad Javad Eslamizade, M.Sc, 4,5Narges Hosseinmardi, Ph.D, 1Mahyar Janahmadi, Ph.D, 1,*
Neuroscience Research Center and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Neuroscience Research Center, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran
Department of Biology, Shahid Chamran University, Ahvaz, Iran
Shefa Neuroscience Research Center, Khatam Al Anbia Hospital, Tehran, Iran
Department of Neuroscience, School of Advanced Medical Technology, Iran University of Medical Sciences, Tehran, Iran
Neuroscience Research Center and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Neuroscience Research Center, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran
Department of Biology, Shahid Chamran University, Ahvaz, Iran
Shefa Neuroscience Research Center, Khatam Al Anbia Hospital, Tehran, Iran
Department of Neuroscience, School of Advanced Medical Technology, Iran University of Medical Sciences, Tehran, Iran
*Corresponding Address: P.O.Box: 19615-1178 Neuroscience Research Center and Department of Physiology Medical School Shahid Beheshti University of Medical Sciences Evin Tehran Iran Email:Janahmadi@sbmu.ac.ir
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Abstract

Objective

Resveratrol, a phytoalexin, has a wide range of desirable biological actions. Despite a growing body of evidence indicating that resveratrol induces changes in neu- ronal function, little effort, if any, has been made to investigate the cellular effect of res- veratrol treatment on intrinsic neuronal properties.

Materials and Methods

This experimental study was performed to examine the acute effects of resveratrol (100 µM) on the intrinsic evoked responses of rat Cornu Ammonis (CA1) pyramidal neurons in brain slices, using whole cell patch clamp re- cording under current clamp conditions.

Results

Findings showed that resveratrol treatment caused dramatic changes in evoked responses of pyramidal neurons. Its treatment induced a significant (P<0.05) increase in the after hyperpolarization amplitude of the first evoked action potential. Resveratrol-treated cells displayed a significantly broader action potential (AP) when compared with either control or vehicle-treated groups. In addition, the mean instantaneous firing frequency between the first two action potentials was significantly lower in resveratrol-treated neurons. It also caused a significant reduction in the time to maximum decay of AP. The rheobase current and the utilization time were both significantly greater following resveratrol treatment. Neurons exhibited a significantly depolarized voltage threshold when exposed to resveratrol.

Conclusion

Results provide direct electrophysiological evidence for the inhibitory effects of resveratrol on pyramidal neurons, at least in part, by reducing the evoked neural activity.