Past Issue

Volume 12, Supplement 1,Winter 2011 (Presented at The 1st International Student Congress) Pages: 52-53

P-29: CCR-4 Variations at C1014T Position in Patients with Breast Cancer

Objective: CCR-4 is a CC chemokine receptor, playing a critical role for regulating immune balance and potentially expresses on Th2 and Treg cells. It has been suggested that CCR-4 ligation with its ligand; CCL-22, participates in the migration of Treg cells to the site of primary breast tumors. Accumulation of Treg as well as Th2 lymphocytes has already been indicated to be associated with poor prognosis in Breast cancer patients. We aimed, in the present study, to examine the association of CCR-4 gene polymorphism at position 1014 C/T with Iranian women susceptibility to breast carcinoma. Materials and Methods: One hundred sixty two patients with pathologically confirmed Breast cancer (mean age 49 ± 11) and 153 age-matched healthy women with no personal and familial history of cancer or autoimmune disease (mean age: 51 ± 12) were enrolled to the study. Genotypes were investigated by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. Personal information of the enrolled subjects was collected by questioner and pathological information of the patients was obtained from patient’s files. Statistical analyses were performed by SPSS and EPI-INFO software packages. And Hardy-Weinberg equilibration was calculated by using Arlequin 3.1 a population genetics software package. Results: Arlequin analysis showed no deviation from Hardy-Weinberg equilibrium among both patients and controls. The frequencies of CC, CT and TT genotype in patients and control group were 90 (55.6%) vs 84(54.9%), 55 34% vs 59( 38.6%) and 11( 6.8% )vs10( 6.5%) respectively. In addition, the frequencies of C and T allels in patient and control groups were 75.32 % vs 74.18 % and 24.68 % vs 25.81% respectively. Statistical analysis revealed no significant differences in the frequencies of genotype and alleles between patients and controls (p=0.83 and p=0.81 respectively). Conclusion: It has been already published that the C to T transition at position 1014 in CCR4 genes is affected mRNA stability, which consequently might change the final protein level. There are no already published data on cancer; however a study in Japanese patients showed no significant association between 1014 C/T SNP of CCR4 and susceptibility to atopic dermatitis. As the first study to investigate CCR-4 genetic markers in cancer we were notable to indicate the association of CCR-4 1014 C/T polymorphism with susceptibility of Iranians to breast cancer