P-40: Co culture of Human Umbilical Cord Mesenchymal Stem Cells and Glioblastoma Cells


Kaviani M *, Yari N , Mahdinia Z , Eskandary H , Nematollahi-Mahani SN ,

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Objective: There is currently no optimal treatment for glioblastoma multiforme; the most common malignant brain tumor in adults, and patient typical survive < 1 year. This poor outcome relates at least in part to the inability to deliver therapeutic agents to the tumor. Recent evidence suggests that stem cells may inhibit proliferation of malignant cells. Human umbilical cord mesenchymal stem cells can be easily obtained and proliferate rapidly in culture. hUCMs are immunologically compatible and amenable to stable transfection. The aim of this study was to determine whether hUCMs can effectively inhibit expansion of human gliomas in a coculture system. Materials and Methods: hUCMs harvested from human umbilical cord were propagated in laboratory. hUCMs and U87 cells were plated at a density of 5×104 in 50 µl <font><font>drop</font></font> of the basic culture medium, 1 cm apart from each other. The cells were given one day to attach to the substratum. After one day, basic culture medium containing DMEM / F12 supplemented with 10% FBS, 100U/ml penicillin- streptomycin was added. Cultures were maintained at 37 º C in a humidified atmosphere containing 5% CO2. The invasion of U87 cells in various directions was measured by a software (analysis@) in micrometer and the data were compared according to the migration of U87 cells in different directions. Results: Proliferation of U87 cells was less pronounced in the vicinity to the hUCM cells. The U87 cells at the vicinity of hUCMs have migrated 153.96 µm while in three other directions they have migrated 212.25 µm, 264.05 µm and 288.72 µm. Conclusion: hUCMs can inhibit the spread of U87 glioma cells, whether inhibition of the growth of U87 cells by hUCM cells has therapeutic advantages needs to be explored