P-55: Evaluation of Endothelial Nitric Oxide Synthase VNTR (Intron 4 a/b) Polymorphism Association with Diabetic Neuropathy (Pages: 64-65)

Mehrab-Mohseni M *, Amoli MM, Amiri P , Sayahpour FA , Tavakkoly Bazzaz J , Hasani-Ranjbar Sh ,


Objective: Endothelial derived nitric oxide (NO) has an important role in development of diabetic complications.Since in vitro studies imply that treatment of endothelial cells with a high concentration of glucose results in both up-regulation of the NOS gene and increased activity of NO, it indirectly proposes that the increased breakdown of NO might be the case in diabetes, probably due to other metabolic disorders of diabetes, rather than hyperglycaemia itself. The aim of this study was to examine the association between eNOS intron 4 VNTR polymorphism and diabetic neuropathy in an Iranian population. Materials and Methods: Diabetic neuropathy (DNU) was defined by symptoms or signs according to Diabetes Control and Complication Trial criteria. The study group comprised of type 2 diabetes patients with DNU (n=146) who were recruited from diabetes clinic in the Aliebn Abitaleb hospital, Rafsanjan University, South-east of Iran. Normal healthy controls (n=96) were recruited from the same area.All the subjects selected for this study were from Fars origin. DNA was extracted from WBCs, using salting-out method.PCR was performed to determine allele and genotype frequencies for eNOS gene VNTR polymorphism. Results: We found that in patients with diabetic neuropathy the frequency of a allele was significantly increased compared to the controls p=0.03, OR=1.8 95%CI (1.00-3.7). Conclusion: We found an association between eNOS gene intron VNTR (4 a/b) polymorphim and risk of DNU. people who carry a allel are more susceptible to DNU