Objective: The CC chemokine receptor 5 (CCR5) delta 32 allele results in a nonfunctional form of the chemokine receptor and has been implicated in a variety of immune-mediated diseases. CCR5Δ32 allele may predispose to chronic liver disease. Here, this study was undertaken to investigate the association between susceptibility to HBV infection and CCR5 polymorphisms in HBV infected cases and healthy controls. Materials and Methods: Between October 2009 and November 2010, a total of 486 Iranian persons were enrolled into two different groups; HBV infected cases (n=257), who were HBsAg-positive, and healthy controls (n=229). We assessed polymorphisms in CCR5 gene using specific CCR5 oligonucleotide primers surrounding the breakpoint deletion, detected a 182 bp product from the normal CCR5 allele and a 150 bp product from the 32 bp deletion allele. Genotype distributions of the HBV infected cases and healthy controls were determined and compared. Results: The CCR5/CCR5 genotype was found in 252 individuals (98%) 0f HBV infected cases and 197 individuals (86%) 0f healthy controls. Only 5 (2%) and 9 (3.9%) of HBV infected cases and healthy controls carried the heterozygous genotype, respectively. None of HBV infected cases carried the CCR5Δ32 homozygous genotype while 23 of healthy controls (10%) carried the CCR5 delta 32 homozygous genotype (P value= 0.0001). 449 of 486 (92.4%) carried the CCR5/CCR5 genotype while 23 of them (4.7%) carried the CCR5 delta 32 homozygous genotype (p value= 0.0001). Conclusion: No association was seen between susceptibility to HBV infection and CCR5Δ32 polymorphism. Further studies are needed to clarify the effect of CCR5Δ32 polymorphism on susceptibility to HBV infection.