P-64: Relationship Study of Six SNPs in PRM1, PRM2 and TNP2 Genes with Idiopathic Azoospermia in Iranian Infertile Men (Pages: 68-69)

Siassi E *, Aleyasin A , Mowla SJ ,


Objective: Histones are replaced by protamines, undergoes substantial condensation to package DNA into the sperm head during mammalian spermatogenesis. Protamine genes defects have been reported to cause sperm DNA damage and male infertility. In this study relationship among some protamines genes family SNPs include PRM1 (C321A), PRM2 (C248T) and TNP2 (T1019C), (G1272C), (1036 and 1046 bp G del) were studied in 96 idiopathic infertile men with azospermia and oligospermia and 100 normal control men. Materials and Methods: Analysis of SNPs were performed using restriction fragment length polymorphism (PCR-RFLP), single strand conformational polymorphism (PCR-SSCP) and PCR sequencing. Results: The prevalence of tested SNPs were zero in both infertile patients and fertile control groups except for PRM1 (C321A) and TNP2 (G1272C) in which frequency of altered AA and GG genotypes were slightly higher in case group. However, statistical analysis showed no significant results P> 0.05 for both loci. Conclusion: These results are consistent with previous studies and indicating that all tested SNPs were not associated with oligospermia and azospermia have occurred due to idiopatic male infertility in Iranian population