Objective: Mature dendritic cells (DCs) are the most important cells that have a key role in the initiation of immune response. The use of maturation factors in DC differentiation provides a promising approach in immunotherapy.In this study, we compared four maturation factors included: tumor necrosis factor-α (TNF-α), polyribocytidylic acid, lipopolysacharide (LPS), CpG oligonucleotides in inducing DC maturation. Materials and Methods: We generated immature DCs with GM-CSF in combination with IL-4 from peripheral blood mononuclear adherent cells and use TNF-α, polyribocytidylic acid, LPS and CpG oligonucleotides for the induction of DC maturation. CD83 maturation marker on the DC was analyzed by flowcytometry after 7 days. In addition, proliferation of CD4 T-cell evaluated by MTT assay. Results: The results demonstrated a comparable high level of CD83 expression on the mature DCs generated by maturation factors treatment of the DCs. However, a significantly poorer proliferation of lymphocyte cultured with the polyribocytidylic acid-treated DCs was observed compared to the CpG-treated DCs in mixed leukocyte reaction(p=0.026). Conversely, a significantly stronger proliferation of lymphocytes was observed when culture with TNF-α-treated DCs compared to the LPS-treated DCs(p=0.025). Conclusion: Our findings indicated that all of maturation inducing factors can be used in DC maturation but TNF-α and CpG were the preferred in vitro maturation factors. It is concluded that maturation of DCs by these factors can be used to produce effective immune responses.