P-73: Reactive Oxygen Species Encapsulated in Magnetically Guided Nanoparticles and Microspheres to Overcome the Common Complication of Multidrug Resistance in Treatment of Cancer: Potential Alternative for Imunotherapy of solid tumors
Objective: Cellular oxidative state is a vital factor for the survival of cells. Reactive Oxygen Species (ROS) are known to be produced by all cells as a by-product of cellular respiration. Excessive intracellular ROS results in lipid peroxidation and high extracellular concentrations are known to cause apoptosis in human cells. As ROS is highly reactive and destructive in most cells, excessive amounts may be collected from cells and disposed into cancer cells or infectious sites that need to be destroyed. Materials and Methods: Construction of precisely designed nanoparticles and microspheres is a promising method to transport highly reactive species through the body in Treatment of Cancer, which can possibly be acquired by the cells. The potential of such applications are to be discussed in the content of this paper. Results: Since cytoplasm ROS concentrations are elevated in cancer cells due to up-regulation of genes encoding for trans-membrane proteins such as adenosine triphosphate (ATP)- binding cassete (ABC) family, they are more prone to fluctuations in ROS concentration. The over-expression special protein transporters located in the cell membrane result in cellular based Multidrug resistance (MDR). MDR is a condition that can develop in any cell as a result of chronic exposure to non-toxic levels of a therapeutic agent. Conclusion: As the affectivity of chemotherapy is limited by MDR, extensive research is taking place aiming to replace chemotherapeutics which produce severe side effects with natural safer alternatives. However, the transportation of such reactive agents in the body is limited by their high molecular weight, hy<font><font>drop</font></font>hilicity, structural fragility, and complexity. To overcome these hurdles, stabilized ROS can be transported via magnetically guided nanoparticle to be unloaded locally and penetrated into the cancer cells to kill the tumor. Unlike immunotherapy, which relies solely on the body's immune system and its capabilities, nano- microcarriers allow the usage of the entire body resources to repair one particular defective site.