P-78: Association Between TIM-1 Gene Polymorphisms with Allergic Rhinitis and Serum Immunoglobulin Levels
Objective: Allergic Rhinitis is caused by the interaction of multiple genetic and environmental factors. One members of the T cell immunoglobulin domain and mucin domain (TIM) gene family, TIM-1, is located in the chromosome 5q31-33 region. This molecule has been involved in T-cell differentiation and implicated in allergic diseases. The aim of present study is to analyze the association between TIM-1 gene polymorphisms with susceptibility to allergic rhinitis, Serum IgE and IgA Levels in Iranian population. Materials and Methods: In a case–control study, 122 allergic rhinitis patients and 130 allergy-free controls were recruited according to age and gender. We investigated two TIM-1 promoter single nucleotide polymorphisms (SNPs), −416G>C (rs9313422) and -1454G>A (rs41297579), by using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Additionally, the relationship between this SNPs and serum IgE and IgA levels in this population was also evaluated; Serum total IgE was measured by Enzyme-linked immunosorbent assay (ELISA) and Serum total IgA was measured by using Neflometry system. Statistical analysis was conducted with SPSS16.0 software. Results: We found that the -416G>C and -1454G>A SNPs were significantly associated with allergic rhinitis susceptibility in our study population (odds ratio 2.73, 95% confidence interval 1.59-7.21; p=0.001 for-416) and (odds ratio 1.91, 95% confidence interval 1.29-3.12; p=0.003 for-1454). In addition to, the serum IgE and IgA levels in the allergic rhinitis subgroup with TIM-1 gene polymorphisms were significantly higher than the allergic rhinitis subgroup without this SNPs and the control group (p<0.05) . Conclusion: Results suggest that the TIM-1 gene polymorphisms (-416G>C and -1454G>A) are associated with the susceptibility of allergic rhinitis and serum IgE and IgA levels, also has provided genetic data on TIM-1 gene in Iranian population and a basis for searching immune-mediated disease-related TIM-1 haplotype.