Objective: Hepatitis B Virus is the most common reason of chronic and acute liver diseases all over the world. Several studies have reported cytokines genes are associated with HBV chronicity and/or persistence or disease severity. Interleukin-1 is one of the most pro-inflammatory agents. The most important members of the IL-1 family are the IL-1α, IL-1β and IL-1 receptor antagonists (IL-1ra). Studies have shown that there is a relationship between polymorphisms of IL-1β and IL-1RNgenes and susceptibility to chronic hepatitis B. This is the first study report about relationship between IL-1α gene and Hepatitis. This study is to investigate the relationship between the polymorphisms of IL-1A gene and susceptibility to chronic hepatitis B. Materials and Methods: Genomic DNA was extracted from the peripheral blood of 213 patients with chronic hepatitis B (161 male, 52 female aged 11-67, mean = 35.17 ± 11.97) and 291 normal control (162 male, 129 female aged 18-62, mean = 36.46 ± 8.43) who referred to Cell & Molecular Research Center of Gorgon during 3 years (86-89) and then subjected to SSP-PCR amplification. PCR products were resolved on a 2% agarose gel and stained with ethidium bromide. STATA software and chi square test was used for statistic calculations. Results: The frequency of three genotype in this locus (CC, TT, CT) in chronic cases were 37.09%, 25.35%, 37.56% and in controls were 36.55%, 9.31% and 54.48% respectively. There was significant excess frequency in genotype TT in cases and CT in controls (p<0.001, OR (TT/CT) = 3.95, 95%CI = 2.314-6.741). Total frequencies of C and T allele were 55.87% and 44.13%in cases and 63.57% and 36.42% in controls, respectively that had no significant relation with Chronic Hepatitis B (p=0.081). Conclusion: There is an association between IL-1A-899 T/T genotype with the chronicity of HBV infections. It is proposed to compare polymorphism of IL-1 gene family between chronic and cleared cases of HBV infections.