P-85: Molecular Cloning and Expression of Recombinant Human Interleukin-7


Sadeghi I *, Ahmadian N , Bahadori Z , Ghalandari M , Behmanesh M ,

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Objective: Human interleukin- (IL-7) is a member of interleukin family that regulates the development and survival of lymphoid and myeloid cells. Recent clinical studies of IL-7 for treatment of various diseases, including immunodeficiency disorders and malignancies have shown promising results suggesting that IL-7 may facilitate additional therapeutic endeavors including bone marrow/organ transplantation and cancer immunotherapy, hence, it is considered to be produced in large amounts using prokaryotic and eukaryotic expression systems. Previously, a study reported that could be purified from inclusion bodies using a prokaryotic system, however, the required refolding step limits the recovery rates. Currently human IL-7 is produced from E. coli expression system which involves inclusion bodies that required refolding steps Materials and Methods: : Using specific primers, the desired fragment of human IL-7 gene was amplified by PCR, its cDNA has been made and amplified and was cloned into the PET32a and then transformed into the BL21. Results: The recombinant human IL-7 was successfully amplified, transformed, cloned and expressed in BL21. Conclusion: The recombinant human IL-7 can be easily cloned and expressed in prokaryotic systems